In Vitro Binding and Autoradiographic Localization of Human Chorionic Gonadotropin and Follicle Stimulating Hormone in Rat Testes during Development

摘要: The concept that certain target tissues contain specific recep­tors for peptide hormones has been widely recognized (1). In almost all instances the cellular attachment of to cell surface correlated with activation mem­brane bound adenylate cyclase and production 3′, 5′ cyclic adenosine monophosphate, presumed intracellular mediator hormone action A similar sequence events postulated occur after testicular interstitial tissue was exposed human chorionic gonadotropin (hCG) or lutenizing (LH) (2, 3), seminiferous epithelial cells were follicle stimulating (FSH) (4). While these results support notion hCG/LH FSH exert their effects on in tubular compartments testis, positive identification sites high-affinity binding have not defined at level same testis. Within this framework, it should be radioactive LH localized selectively Leydig (5–7) ferritin-labeled appeared tubule wall cytoplasm Sertoli rat testes (8). However, latter findings may ques­tioned view possibility metabolites labeled hor­mone rather than per se , since used highly impure massive amounts (i.e., 5 10 mg) injected Recent advances autoradio-graphic localization circum­vent problems associated (9) prompted us reinvestigate gonadotrophic hor­mones