New Approaches for Carbon Nanotubes-Based Biosensors and Their Application to Cell Culture Monitoring
作者： C. Boero , J. Olivo , G. De Micheli , S. Carrara
DOI: 10.1109/TBCAS.2012.2220137
关键词:
摘要: Amperometric biosensors are complex systems and they require a combination of technologies for their development. The aim the present work is to propose new approach in order develop nanostructured real-time detection multiple metabolites cell culture flasks. fabrication five Au working electrodes onto silicon substrate achieved with CMOS compatible microtechnology. Each electrode presents an area 0.25 mm2, so structuration carbon nanotubes specific functionalization carried out by using spotting technology, originally developed microarrays DNA printing. characterized cyclic voltammetry compared commercially available screen-printed electrodes. Measurements under flow conditions, simple fluidic system guarantee continuous next functionalized different enzymes calibrated glucose, lactate, glutamate. Finally, some tests performed on surnatant conditioned medium sampled from neuroblastoma cells (NG-108 line) detect glucose lactate concentration after 72 hours cultivation. biosensor online shows very promising results towards circuits monitoring.
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