Electrochemical sensor array for bioprocess monitoring
作者： M. Bäcker , L. Delle , A. Poghossian , M. Biselli , W. Zang
DOI: 10.1016/J.ELECTACTA.2011.04.030
关键词:
摘要: Abstract A silicon-based biosensor chip consisting of an array amperometric enzyme sensors has been developed for monitoring the concentration glutamate and glutamine in cell-culture fermentation processes. The sensor was constructed by immobilising oxidase, while sequential coupling glutaminase oxidase. enzymes were immobilised means cross-linking with glutaraldehyde on surface patterned platinum thin-film electrodes. biosensors have electrochemically characterised solutions different concentrations terms sensitivity, response time, linear working range lifetime. high sensitivity 96 nA/mM 100 nA/mM registered sensors, respectively. preliminary experiments medium shown a good correlation between measured commercially available biochemistry analyser. obtained results demonstrate feasibility realised
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I Moser, G Jobst, E Aschauer, P Svasek, M Varahram, G Urban, VA Zanin, GY Tjoutrina, AV Zharikova, TT Berezov, Miniaturized thin film glutamate and glutamine biosensors. Biosensors and Bioelectronics. ,vol. 10, pp. 527- 532 ,(1995) , 10.1016/0956-5663(95)96928-R
M.J. Sch�ning, H. L�th, Novel Concepts for Silicon‐Based Biosensors Physica Status Solidi (a). ,vol. 185, pp. 65- 77 ,(2001) , 10.1002/1521-396X(200105)185:1<65::AID-PSSA65>3.0.CO;2-Y
Niko Näther, David Rolka, Arshak Poghossian, Milena Koudelka-Hep, Michael J. Schöning, Two microcell flow-injection analysis (FIA) platforms for capacitive silicon-based field-effect sensors Electrochimica Acta. ,vol. 51, pp. 924- 929 ,(2005) , 10.1016/J.ELECTACTA.2005.04.066
M. Bäcker, S. Pouyeshman, Th. Schnitzler, A. Poghossian, P. Wagner, M. Biselli, M. J. Schöning, A silicon‐based multi‐sensor chip for monitoring of fermentation processes Physica Status Solidi (a). ,vol. 208, pp. 1364- 1369 ,(2011) , 10.1002/PSSA.201001139
M. Bäcker, S. Beging, M. Biselli, A. Poghossian, J. Wang, W. Zang, P. Wagner, M.J. Schöning, Concept for a solid-state multi-parameter sensor system for cell-culture monitoring Electrochimica Acta. ,vol. 54, pp. 6107- 6112 ,(2009) , 10.1016/J.ELECTACTA.2009.02.091
Th. Schalkhammer, Ch. Lobmaier, B. Ecker, W. Wakolbinger, E. Kynclova, G. Hawa, F. Pittner, Microfabricated glucose, lactate, glutamate and glutamine thin-film biosensors Sensors and Actuators B-chemical. ,vol. 19, pp. 587- 591 ,(1994) , 10.1016/0925-4005(93)01094-K
Carles Campmaj�, Jordi Joan Cair�, Anna Sanfeliu, Esteve Mart�nez, Salvador Alegret, Francesc G�dia, Determination of ammonium and L-Glutamine in hybridoma cell cultures by sequential flow injection analysis Cytotechnology. ,vol. 14, pp. 177- 182 ,(1994) , 10.1007/BF00749614
Ch. Lobmaier, Th. Schalkhammer, G. Hawa, B. Ecker, F. Pittner, Photostructurized electrochemical biosensors for bioreactor control and measurement in body fluids. Journal of Molecular Recognition. ,vol. 8, pp. 146- 150 ,(1995) , 10.1002/JMR.300080125
S. Alison Arnold, John Crowley, Nigel Woods, Linda M. Harvey, Brian McNeil, In-situ near infrared spectroscopy to monitor key analytes in mammalian cell cultivation. Biotechnology and Bioengineering. ,vol. 84, pp. 13- 19 ,(2003) , 10.1002/BIT.10738
Arshak Poghossian, Michael J. Schöning, Detecting Both Physical and (Bio‐)Chemical Parameters by Means of ISFET Devices Electroanalysis. ,vol. 16, pp. 1863- 1872 ,(2004) , 10.1002/ELAN.200403074