HLAMatchmaker: a molecularly based algorithm for histocompatibility determination. I. Description of the algorithm.

摘要: This report describes an algorithm for identifying acceptable HLA antigens highly alloimmunized patients without the need extensive serum screening. is based on concept that immunogenic epitopes are represented by amino acid triplets exposed parts of protein sequences human leukocyte antigen chains (HLA-A, HLA-B, and HLA-C) accessible to alloantibodies. A computer program (HLAMatchmaker) has been developed determine class I compatibility at molecular level. It makes intralocus interlocus comparisons polymorphic in sequence positions spectrum non-shared donor antigens. In most cases it possible identify certain mismatched share all their with patient's could therefore, be considered fully compatible. HLAMatchmaker permits also identification additional mismatches as determined from triplet information HLA-typed panel cells do not react serum.HLAMatchmaker provides assessment donor-recipient structural level this different conventional methods mere counting numbers or CREGs. selection strategy suitable especially allosensitized a compatible transplant platelet transfusion.