Kits for multiplexed nucleic acid analysis by capture of single-stranded DNA produced from double-stranded target fragments

摘要: A method of fragmentation double stranded DNA is disclosed for use in nucleic acid analysis, notably the multiplexed analysis polymorphisms and mutations. The produces a multiplicity labeled sense anti-sense fragments which are not complementary, thus do significantly re-anneal under conditions suitable hybridization (or capture-mediated elongation analysis) and/or display desired or predicted length distribution. Cleavage sites can be selected such that short, yet long enough to allow discrimination among an assay, as matter statistical probability, majority contain at least one nucleotide facilitate detection.