Concentrating White Blood Cells for DNA Extraction from a Leukodepleted Blood Sample
作者： Sukanta Banerjee
DOI:
关键词: Volume (thermodynamics) 、 DNA extraction 、 Biology 、 Whole blood 、 Sample (material) 、 Reagent 、 Lysis 、 Centrifugation 、 Phosphate buffered saline 、 Chromatography
摘要: Reagents and a method for the pre-concentration of WBCs from leukodepleted blood segment samples are described. The reagent comprises: lytic reagent, example, saponin in phosphate buffered saline(PBS), wherein amount is range about 1% to 10% percent (w/w). comprises: contacting specific volume leukoreduced sample with regent; mixing two so as selectively lyse RBCs whole sample; subjecting mixture centrifugation, separate into WBC rich phase lysed-RBC phase; discarding supernatant containing lysed RBC resuspending PBS.
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