Lytic reagent composition for leukocyte differential analysis
作者： Harold Richardson Crews , Michael N. Elliott , II James Harrison Carter
DOI:
关键词: Flow cytometry 、 Lysis 、 Light absorbance 、 Lytic cycle 、 Alkyl 、 Chromatography 、 Reagent 、 Differential analysis 、 Composition (visual arts) 、 Chemistry
摘要: A lytic reagent composition and the method of use for differential analysis leukocytes using flow cytometry are disclosed. The includes a short chain alkyl oxyethanol, such as 2-methoxyethanol, 2-ethoxyethanol, 2-propoxyethanol, or 2-isopropoxyethanol, in sufficient amount to preserve leukocytes; non-lysing nonionic surfactant debris solublizer; an inorganic buffer maintain pH range from 9.1 10.7. is used lyse red blood cells leukocytes, well sheath focus measurement. composition, when on cytometric analyzer with multiple angle light scatter absorbance measurements, enables differentiation into five subpopulations.
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