Ribosome-inactivating proteins from the seeds of Saponaria officinalis L. (soapwort), of Agrostemma githago L. (corn cockle) and of Asparagus officinalis L. (asparagus), and from the latex of Hura crepitans L. (sandbox tree)

摘要: Ribosome-inactivating proteins, similar to those already known [Barbieri & Stirpe (1982) Cancer Surveys 1, 489-520] were purified from the seeds of Saponaria officinalis (two proteins), Agrostemma githago (three and Asparagus latex Hura crepitans (one protein). The yield ranged 8 400 mg/100 g starting material. All proteins have an Mr approx. 30000 alkaline isoelectric point. Their sugar content varies 0 (proteins S. officinalis) 40% (protein H. crepitans). ribosome-inactivating inhibit protein synthesis by rabbit reticulocyte lysate, ID50 (concentration giving 50% inhibition) ranging 1 ng/ml (a 18 A. githago). Those which tested (the githago) also polymerization phenylalanine isolated ribosomes, acting in apparently catalytic manner. inhibited HeLa cells, with 4 micrograms/ml, whereas had more than 50-100 micrograms/ml. reduced number local lesions tobacco-mosaic virus leaves Nicotiana glutinosa.