Differential gene expression analysis related to extracellular matrix components in drug-resistant RPMI-8226 cell line.

摘要: Drug resistance remains a major obstacle to the successful use of chemotherapeutic drugs for many types cancers including multiple myeloma. It is becoming increasingly apparent that tumor microenvironment could provide shelter malignant plasma cells allow their survival after initial drug exposure. This study demonstrates alterations in gene expression levels several extracellular matrix (ECM) components prednisone, vincristine and melphalan-resistant RPMI-8226 myeloma cells. Resistant were developed through stepwise selection by increasing concentrations drugs. Microarray analysis was carried out genes up- or downregulated more than two-folds considered as significant. Different ECM altered different resistant sublines. ITGAL ITGB2 both overexpressed cell line whereas they prednisone subline. On other hand, LAMC1 drastically subline it its melphalan variant. FN1 only upregulated However, COL21A1 which an component blood vessel walls, all ADAM17 report provides preliminary vitro relationship between Since drug-resistant sublines do not have similar cells, correlation proteins with requires further analysis.