Protection against malaria by Plasmodium yoelii sporozoite surface protein 2 linear peptide induction of CD4+ T cell- and IFN-gamma-dependent elimination of infected hepatocytes.

摘要: Plasmodium falciparum sporozoite surface protein 2 (SSP2), also known as TRAP, is included in experimental human malaria vaccines because yoelii SSP2 the target of protective CD8+ CTL that eliminate P. yoelii-infected hepatocytes mice. We now report immunization with a synthetic branched-chain peptide including four copies PySSP2 sequence, NPNEPS, and two tetanus toxin T helper epitopes adjuvant TiterMax, or an 18 amino acid (NPNEPS)3 protects A/J, but not BALB/c C57BL/6 Transfer lymphocyte-enriched immune splenocytes naive mice; vivo depletion CD4+ cells eliminates vaccine-induced protection; treatment anti-IFN-gamma reverses activity against infected hepatocytes. Lymph node from immunized BALB/c, mice recognize vitro. However, protected A/J respond predominantly Th1 pattern lymphocyte response, non-protected strains Th2 pattern. There are many examples transferring protection infectious organisms. to our knowledge, this first formal demonstration linear induces cell-dependent, IFN-gamma dependent, genetically restricted sterile immunity agent.