Bruton’s Tyrosine Kinase Is Required for TLR2 and TLR4-Induced TNF, but Not IL-6, Production
作者： Nicole J. Horwood , Theresa H. Page , John P. McDaid , Christine D. Palmer , Jamie Campbell
DOI: 10.4049/JIMMUNOL.176.6.3635
关键词:
摘要: Bruton's tyrosine kinase (Btk), the gene mutated in human immunodeficiency X-linked agammaglobulinemia, is activated by LPS and required for LPS-induced TNF production. In this study, we have investigated role of Btk both signaling via another TLR (TLR2) production other proinflammatory cytokines such as IL-1beta, IL-6, IL-8. Our data show that agammaglobulinemia PBMCs, stimulation with TLR4 (LPS) or TLR2 (N-palmitoyl-S-[2, 3-bis(palmitoyloxy)-(2R)-propyl]-(R)-cysteine) ligands produces significantly less IL-1beta than normal controls. contrast, a lack has no impact on IL-8, anti-inflammatory cytokine, IL-10. previous suggested lies within p38-dependent pathway stabilizes mRNA. Accordingly, TaqMan quantitative PCR analysis actinomycin D time courses presented work shows overexpression able to stabilize TNF, but not IL-6 Furthermore, using p38 inhibitor SB203580, TLR4-induced requires activity MAPK. These provide evidence common requirement TLR2- TLR4-mediated induction two important cytokines, reveal differences TLR-mediated signals
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researchgate.net 参考文章(24)
J Han, J D Lee, P S Tobias, R J Ulevitch, Endotoxin induces rapid protein tyrosine phosphorylation in 70Z/3 cells expressing CD14 Journal of Biological Chemistry. ,vol. 268, pp. 25009- 25014 ,(1993) , 10.1016/S0021-9258(19)74564-5
Yukihito Kabuyama, Miwako K. Homma, Tomohiro Kurosaki, Yoshimi Homma, Early signaling events induced by 280-nm UV irradiation European Journal of Biochemistry. ,vol. 269, pp. 664- 670 ,(2002) , 10.1046/J.0014-2956.2001.02698.X
Masahiro Yamamoto, Soh Yamazaki, Satoshi Uematsu, Shintaro Sato, Hiroaki Hemmi, Katsuaki Hoshino, Tsuneyasu Kaisho, Hirotaka Kuwata, Osamu Takeuchi, Koichiro Takeshige, Tatsuya Saitoh, Shoji Yamaoka, Naoki Yamamoto, Shunsuke Yamamoto, Tatsushi Muta, Kiyoshi Takeda, Shizuo Akira, Regulation of Toll/IL-1-receptor-mediated gene expression by the inducible nuclear protein IκBζ Nature. ,vol. 430, pp. 218- 222 ,(2004) , 10.1038/NATURE02738
Sangita Mukhopadhyay, Madhuchhanda Mohanty, Anita Mangla, Anna George, Vineeta Bal, Satyajit Rath, Balachandran Ravindran, None, Macrophage Effector Functions Controlled by Bruton’s Tyrosine Kinase Are More Crucial Than the Cytokine Balance of T Cell Responses for Microfilarial Clearance The Journal of Immunology. ,vol. 168, pp. 2914- 2921 ,(2002) , 10.4049/JIMMUNOL.168.6.2914
Shizuo Akira, Kiyoshi Takeda, Tsuneyasu Kaisho, Toll-like receptors: critical proteins linking innate and acquired immunity Nature Immunology. ,vol. 2, pp. 675- 680 ,(2001) , 10.1038/90609
S.L. Weinstein, J.S. Sanghera, K Lemke, A.L. DeFranco, S.L. Pelech, Bacterial lipopolysaccharide induces tyrosine phosphorylation and activation of mitogen-activated protein kinases in macrophages. Journal of Biological Chemistry. ,vol. 267, pp. 14955- 14962 ,(1992) , 10.1016/S0021-9258(18)42133-3
Alan Aderem, Richard J. Ulevitch, Toll-like receptors in the induction of the innate immune response Nature. ,vol. 406, pp. 782- 787 ,(2000) , 10.1038/35021228
Sarah L. Doyle, Caroline A. Jefferies, Luke A. O'Neill, Bruton's Tyrosine Kinase Is Involved in p65-mediated Transactivation and Phosphorylation of p65 on Serine 536 during NFκB Activation by Lipopolysaccharide Journal of Biological Chemistry. ,vol. 280, pp. 23496- 23501 ,(2005) , 10.1074/JBC.C500053200
Caroline A. Jefferies, Sarah Doyle, Cornelia Brunner, Aisling Dunne, Elizabeth Brint, Claudia Wietek, Eva Walch, Thomas Wirth, Luke A. J. O'Neill, Bruton's Tyrosine Kinase Is a Toll/Interleukin-1 Receptor Domain-binding Protein That Participates in Nuclear Factor κB Activation by Toll-like Receptor 4 Journal of Biological Chemistry. ,vol. 278, pp. 26258- 26264 ,(2003) , 10.1074/JBC.M301484200
Anita Mangla, Anupriya Khare, Varanasi Vineeth, Nagesh Narayan Panday, Asok Mukhopadhyay, Balachandran Ravindran, Vineeta Bal, Anna George, Satyajit Rath, None, Pleiotropic consequences of Bruton tyrosine kinase deficiency in myeloid lineages lead to poor inflammatory responses Blood. ,vol. 104, pp. 1191- 1197 ,(2004) , 10.1182/BLOOD-2004-01-0207