Isolation of endophytic bacteria producing the anti-cancer alkaloid camptothecine from Miquelia dentata Bedd. (Icacinaceae)

摘要: Abstract Camptothecine (CPT), a quinoline alkaloid, is potent inhibitor of eukaryotic topoisomerase I. Because this activity, several semi-synthetic derivatives CPT are in clinical use against ovarian and small lung cancers. Together with its derivatives, the third largest anti-cancer drug world market. produced by plant species belonging to Asterid clade. In recent past, studies have reported production endophytic fungal associates some these species. paper, we report bacteria isolated from Miquelia dentata Bedd. (Icacinaceae). Besides CPT, also 9-methoxy (9-MeO-CPT), culture, independent host tissue. The chemical nature 9-MeO-CPT was determined LC–MS ESI-MS/MS analysis, shown be similar that One bacterial isolates examined, showed indications attenuation through sub-culture. This first bacteria. identity ascertained Gram staining 16s rRNA sequencing. We discuss possible mechanisms might involved synthesis