Immunoaffinity chromatography of enzymes.

摘要: Abstract Immunoaffinity chromatography of enzymes represents an attractive purification technique suitable for one-step and large-scale to homogeneity. Monoclonal polyclonal antibodies can be used equally well. The broad use the is restricted by harsh elution conditions which are often required. efforts overcome these limitations optimize method reviewed, viz. proenzyme purification, as part multienzyme complexes carried out a mild dissociation step, specific substrates effectors, enzyme stabilization, electrophoretical desorption negative adsorbing impurities from crude extract, hypotonic elution. current practice discussed considering antibody selection, optimization conditions, washing steps using different media. Representative examples given various approaches.