Spread of β-lactam-resistant Pseudomonas aeruginosa in a cystic fibrosis clinic

摘要: Summary Background Pseudomonas aeruginosa colonisation of the airways patients with cystic fibrosis (CF) is associated considerable respiratory morbidity. Although segregation colonised from non-colonised to prevent cross-infection has been recommended, there little evidence that such widespread. We observed a high proportion children attending our CF clinic were P was resistant ceftazidime and other β-lactam antibiotics. used two genomic fingerprinting techniques see whether this may have arisen epidemic spread single strain. Methods The prevalence antibiotic susceptibility organisms determined review laboratory reports in case-notes 120 CF. Isolates cultured sputum 65 ceftazidime-resistant aeruginosa. Polymorphisms total bacterial DNA 92 isolates analysed molecular techniques-pulsed-field gel electrophoresis after restriction enzyme digestion assessment flagellin gene polymorphisms by amplification whole digestion. Results (76–7%) , harboured ceftazidime. Only three had never treated results molecular-fingerprinting concordant showed 55 same This strain ceftazidime, azlocillin, imipenem, sensitive tobramycin ciprofloxacin. Interpretation study provides first long-term outbreak centre. suggest careful surveillance resistance centres should be instituted measures cross-infection. believe antipseudomonal monotherapy considered caution.