Mechanisms of Endothelial Cell ATP Depletion after Oxidant Injury

摘要: ABSTRACT: To investigate mechanisms of ATP depletion in human umbilical vein endothelial cells after oxidant injury, we studied the relationship between DNA damage, activation DNA-repairing enzyme poly ADP-ribose polymerase, NAD depletion, and depletion. We found that stress generated with hypoxanthine-xanthine oxidase glucose-glucose resulted profound damage. When were exposed to 25 50 mU/ml xanthine for 60 min, percentage double-stranded was significantly reduced (p<0.05) 15.2 ± 1.2 4.6 0.5%, respectively, compared 75.7 3.9% control cells. glucose 35.0 1.5% 9.9 7.7%, 73.2 2.4% levels declined simultaneously Because polymerase can consume sufficient interfere synthesis, injury when inhibited 3-aminobenzamide nicotinamide. inhibited, remained normal, but not prevented. conclude results damage from this phenomenon is mechanism