Metabolomic charactetization of yeast cells after dehydration stress
作者： Gema López-Martínez , Montse Poblet , Anna Borrull , Nicolás Rozès Roy , Ricardo Cordero-Otero
关键词:
摘要: In this study, we analyzed the metabolite features of yeasts Saccharomyces cerevisiae, Naumovia castellii, and mikatae. The three species are closely related genetically but differ in their tolerance desiccation stress. Specifically, determined whether certain metabolites correlated with cell viability after stress imposition. metabolomics profiles these strains were compared before rehydration. S. mikatae, presence lysine or glutamine during rehydration led to a 20% increase survival whereas dehydration levels both amino acids yeast drastically reduced.
unirioja.es
sci-hub.se 参考文章(23)
Lee Mcalister-Henn, W. Curtis Small, Molecular genetics of yeast TCA cycle isozymes. Progress in Nucleic Acid Research and Molecular Biology. ,vol. 57, pp. 317- 339 ,(1997) , 10.1016/S0079-6603(08)60285-8
Cornelia Amariei, Masaru Tomita, Douglas B. Murray, Kalesh Sasidharan, Rapid DNA, RNA and protein extraction protocols optimized for slow continuously growing yeast cultures. Yeast. ,vol. 29, pp. 311- 322 ,(2012) , 10.1002/YEA.2911
B. Rodríguez-Porrata, D. Carmona-Gutierrez, A. Reisenbichler, M. Bauer, G. Lopez, X. Escoté, A. Mas, F. Madeo, R. Cordero-Otero, Sip18 hydrophilin prevents yeast cell death during desiccation stress. Journal of Applied Microbiology. ,vol. 112, pp. 512- 525 ,(2012) , 10.1111/J.1365-2672.2011.05219.X
James A. Barnett, Karl-Dieter Entian, A history of research on yeasts 9: regulation of sugar metabolism. Yeast. ,vol. 22, pp. 835- 894 ,(2005) , 10.1002/YEA.1249
Ute Roessner, Cornelia Wagner, Joachim Kopka, Richard N. Trethewey, Lothar Willmitzer, Simultaneous analysis of metabolites in potato tuber by gas chromatography-mass spectrometry. Plant Journal. ,vol. 23, pp. 131- 142 ,(2000) , 10.1046/J.1365-313X.2000.00774.X
Juan I Castrillo, Andrew Hayes, Shabaz Mohammed, Simon J Gaskell, Stephen G Oliver, An optimized protocol for metabolome analysis in yeast using direct infusion electrospray mass spectrometry. Phytochemistry. ,vol. 62, pp. 929- 937 ,(2003) , 10.1016/S0031-9422(02)00713-6
Wim de Koning, Karel van Dam, A method for the determination of changes of glycolytic metabolites in yeast on a subsecond time scale using extraction at neutral pH Analytical Biochemistry. ,vol. 204, pp. 118- 123 ,(1992) , 10.1016/0003-2697(92)90149-2
Hélène Simonin, Laurent Beney, Patrick Gervais, Sequence of occurring damages in yeast plasma membrane during dehydration and rehydration: mechanisms of cell death. Biochimica et Biophysica Acta. ,vol. 1768, pp. 1600- 1610 ,(2007) , 10.1016/J.BBAMEM.2007.03.017
Katrin Larsson, Ricky Ansell, Peter Eriksson, Lennart Adler, A gene encoding sn-glycerol 3-phosphate dehydrogenase (NAD+) complements an osmosensitive mutant of Saccharomyces cerevisiae Molecular Microbiology. ,vol. 10, pp. 1101- 1111 ,(1993) , 10.1111/J.1365-2958.1993.TB00980.X
Benjamin Gonzalez, Jean François, Michel Renaud, A rapid and reliable method for metabolite extraction in yeast using boiling buffered ethanol. Yeast. ,vol. 13, pp. 1347- 1355 ,(1997) , 10.1002/(SICI)1097-0061(199711)13:14<1347::AID-YEA176>3.0.CO;2-O