Conformational changes in intact dengue virus reveal serotype-specific expansion
作者： Xin-Xiang Lim , Arun Chandramohan , Xin Ying Elisa Lim , Nirmalya Bag , Kamal Kant Sharma
DOI: 10.1038/NCOMMS14339
关键词:
摘要: Dengue virus serotype 2 (DENV2) alone undergoes structural expansion at 37 °C (associated with host entry), despite high sequence and homology among the four known serotypes. The basis for this differential across strains serotypes is unknown necessitates mapping of dynamics dengue whole viral particles to describe their coordinated motions conformational changes when exposed host-like environments. Here we capture intact two serotypes, DENV1 DENV2, by amide hydrogen/deuterium exchange mass spectrometry (HDXMS) time resolved Forster Resonance Energy Transfer. Our results show temperature-dependent hotspots on DENV2 showing 40 °C but not 37 °C. HDXMS measurement virion in solution offers a powerful approach identify potential epitopes, map virus-antibody complex structure dynamics, test effects multiple host-specific perturbations viruses complexes.
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