MicroRNA‐127 is Downregulated by Tudor‐SN Protein and Contributes to Metastasis and Proliferation in Breast Cancer Cell Line MDA‐MB‐231

摘要: Tudor-SN is a multifunctional protein that highly expressed in multiple cancers including breast cancer. Tudor-SN, as component RNA-induced splicing complex, was recently reported to regulate gene expression microRNA (miRNA)-dependent manner, such let-7, miR-34a and miR-221. However, how associated with cancer development still remains largely elusive. In the present study, we explored role of Stable knockdown endogenous performed on cell line MDA-MB-231 by small hairpin RNA vectors, suppressed vitro migration invasion ability metastatic line. Interestingly, found miRNA regulator according microarray analysis, further identified negatively regulated miR-127, consequently increased proto-oncogene BCL6 which convincing target miR-127. Moreover, overexpression miR-127 reduced proliferation MDA-MB-231. Collectively, our results suggested novel mechanism promoted metastasis cells via downregulating expression. Anat Rec, 296:1842–1849, 2013. © 2013 Wiley Periodicals, Inc.