Induction of astrocyte differentiation by propentofylline increases glutamate transporter expression in vitro: Heterogeneity of the quiescent phenotype

摘要: Reactive astrocytes display decreased glutamate transporters, such as GLT-1, and a result synaptic clearance is impaired. In addition, these activated are immunocompetent release algesic mediators that can sensitize neurons in the spinal cord. Currently, we evaluated effect of propentofylline (PPF), an experimental antiallodynic agent, on phenotype transporter expression astrocytes. Primary astrocyte cultures, which represent with polygonal morphology low GLT-1 expression, were treated for 3 or 7 days 10, 100, 1,000 μM PPF dibutyryl-cAMP (db-cAMP), known inducer expression. dose-dependently induced to mature phenotype, elongated processes stellate shape, well increased GLAST immunoreactivity, similar seen db-cAMP. Real time RT-PCR Western blot analysis clearly demonstrated caused potent dose-dependent induction mRNA protein Importantly, observed increase transporters was found have functional effect, significantly enhanced uptake 100 sensitive dihydrokainate inhibition, suggesting it mediated. Finally, lipopolysaccaride-induced chemokine investigated. Interestingly, able dampen both MCP-1 (CCL2) MIP-2 (CXCL2) from while db-cAMP this These findings suggest capable differentiating homeostatic, competent distinct by © 2006 Wiley-Liss, Inc.