Polymerase chain reaction-based tests for pan-species and species-specific detection of human Plasmodium parasites

摘要: BACKGROUND: There is still a need to improve the sensitivity of polymerase chain reaction (PCR) tests for malaria detect submicroscopic asexual stage Plasmodium infections during early phase and chronic, asymptomatic infection when parasite burden very low. STUDY DESIGN AND METHODS: The inhibitory effect hemoglobin (Hb) on PCR limits volume blood that can be used in PCR-based detection intraerythrocytic parasites. We lysed red cells with saponin reduce Hb concentration extracted nucleic acid and, as result, significantly increased tested by PCR. analytical was determined using whole spiked ring-stage falciparum parasites, its clinical testing film–positive film–negative samples from individuals living an endemic area Ghana. RESULTS: have developed pan-Plasmodium detects all five human species highest two P. falciparum parasites/mL species-specific distinguished between species. Pan-Plasmodium detected 78 (100%) 19 101 (18.81%) Ghana. equally sensitive collected anticoagulated clotted finger stick into capillaries. CONCLUSION: reported date diagnosis malaria-endemic countries.