摘要: Abstract : The vaccination efficacy of DNA encoding autologous rat ErbB-2 (neu) or heterologous human (Her-2) is compared in neu transgenic mice (BALB neuT). Cross reactivity between Her-2 and was tested initially by immunizing normal twice, i.m. with pEFBosGM-CSF pCMVE2TM orpCDneuTM. E2TM neuTM encodes the extracellular (ECD) transmembrane (TM) domains neu, respectively. Immunized were challenged mammary tumor D2F2 expressing (D2F2/E2) (D2F2/neu). All immunized rejected tumors corresponding antigen. There significant cross-protection against non-corresponding ErbB-2, although antibodies demonstrated little cross-reactivity. When neuT which are tolerant to but not delayed spontaneous tumorigenesis. Therefore, cross-reactive antigens mice, only induced protective immunity NeuT mice. This may indicate a lack cross-reactivity critical inhibiting tumor. To enhance immunogenicity an adjuvant sequence Pan DR Reactive Epitope (PADRE) has been cloned into being tested.