DNA Vaccination of Overcome Peripheral Tolerance

作者: Wei-Zen Wei

DOI: 10.21236/ADA411427

关键词:

摘要: Abstract : The vaccination efficacy of DNA encoding autologous rat ErbB-2 (neu) or heterologous human (Her-2) is compared in neu transgenic mice (BALB neuT). Cross reactivity between Her-2 and was tested initially by immunizing normal twice, i.m. with pEFBosGM-CSF pCMVE2TM orpCDneuTM. E2TM neuTM encodes the extracellular (ECD) transmembrane (TM) domains neu, respectively. Immunized were challenged mammary tumor D2F2 expressing (D2F2/E2) (D2F2/neu). All immunized rejected tumors corresponding antigen. There significant cross-protection against non-corresponding ErbB-2, although antibodies demonstrated little cross-reactivity. When neuT which are tolerant to but not delayed spontaneous tumorigenesis. Therefore, cross-reactive antigens mice, only induced protective immunity NeuT mice. This may indicate a lack cross-reactivity critical inhibiting tumor. To enhance immunogenicity an adjuvant sequence Pan DR Reactive Epitope (PADRE) has been cloned into being tested.

参考文章(28)
Brigid Hogan, Elizabeth Lacy, Frank Costantini, Manipulating the mouse embryo: A laboratory manual ,(1986)
Catherine A. O'Brian, Constantin G. Ioannides, Karen R. Gravitt, J. Taylor Wharton, Bryan Fisk, James L. Murray, Brett W. Anderson, Andrzej P. Kudelka, Identification of Naturally Processed Human Ovarian Peptides Recognized by Tumor-associated CD8+ Cytotoxic T Lymphocytes Cancer Research. ,vol. 57, pp. 87- 93 ,(1997)
Roger Moe, Mary L. Disis, Ann E. Murphy, Robert B. Livingston, Margit Jeschke, Elaine McGlynn, Wei Chen, Martin A. Cheever, Emanuel Calenoff, Graham McLaughlin, Nick Lydon, Bernd Groner, Existent T-cell and antibody immunity to HER-2/neu protein in patients with breast cancer. Cancer Research. ,vol. 54, pp. 16- 20 ,(1994)
Shari A Pilon, Marie P Piechocki, Wei-Zen Wei, None, Vaccination with Cytoplasmic ErbB-2 DNA Protects Mice from Mammary Tumor Growth Without Anti-ErbB-2 Antibody The Journal of Immunology. ,vol. 167, pp. 3201- 3206 ,(2001) , 10.4049/JIMMUNOL.167.6.3201
C. T. Guy, M. A. Webster, M. Schaller, T. J. Parsons, R. D. Cardiff, W. J. Muller, Expression of the neu protooncogene in the mammary epithelium of transgenic mice induces metastatic disease Proceedings of the National Academy of Sciences of the United States of America. ,vol. 89, pp. 10578- 10582 ,(1992) , 10.1073/PNAS.89.22.10578
Wei‐Zen Wei, Wei‐Ping Shi, Anne Galy, Darcy Lichlyter, Sonia Hernandez, Bernd Groner, Lance Heilbrun, Richard F Jones, None, Protection against mammary tumor growth by vaccination with full-length, modified human ErbB-2 DNA. International Journal of Cancer. ,vol. 81, pp. 748- 754 ,(1999) , 10.1002/(SICI)1097-0215(19990531)81:5<748::AID-IJC14>3.0.CO;2-6
Shiquan Liu, Wenjing Liu, John L. Jakubczak, Gregory L. Erexson, Kenneth R. Tindall, Richard Chan, William J. Muller, Sankar Adhya, Susan Garges, Glenn Merlino, Genetic instability favoring transversions associated with ErbB2-induced mammary tumorigenesis Proceedings of the National Academy of Sciences of the United States of America. ,vol. 99, pp. 3770- 3775 ,(2002) , 10.1073/PNAS.052710299
Teresa A. Rose-Hellekant, Kennedy Gilchrist, Eric P. Sandgren, Strain Background Alters Mammary Gland Lesion Phenotype in Transforming Growth Factor-α Transgenic Mice American Journal of Pathology. ,vol. 161, pp. 1439- 1447 ,(2002) , 10.1016/S0002-9440(10)64419-7