Original Article Optimization of medium composition of laccase production by Aspergillus nidulans KF974331 through Response surface methodology- A statistical approach

作者: K. E. Vivekanadan , R. Satish Kumar , T. Balasubramian , S. Kumaresan

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摘要: Laccase producing Aspergillus nidulans CASVK3 was isolated from mangrove sediments were identified through 18S rRNA gene sequences. The medium components and culture conditions for laccase production statistically optimized by Plackett-Burman design. design used to find out the optimal constituents enhance production. Central composite (CCD) revealed that variables such as sucrose (20g/l), peptone (10g/l), guaiocol (3M) Nacl (10g/l) influenced of enzyme. enzyme obtained experimentally coincident with predicted value, enhancement 219.76 U/ml 367.68.68 achieved Response Surface Methodology (RSM).

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