Hypotensive effects of hydrogen sulfide via attenuating vascular inflammation in spontaneously hypertensive rats

摘要: OBJECTIVE To investigate the effects of hydrogen sulfide (H2S) on vascular inflammation and blood pressure in spontaneously hypertensive rats (SHR). METHODS Four weeks old male SHR were treated with saline (control, n = 7), sodium hydrosulfide (NaHS, a H2S donor, 7) propargylglycine (PPG, endogenous production inhibitor, 6) for 5 weeks. Age-natched Wistar Kyoto (WKY) served as normotensive controls (n 8). Five later, systolic (SBP) was measured conscious quiet by means standard tail-cuff method. The protein expressions intercellular adhesive molecule-1 (ICAM-1), nuclear transcriptional factor-kappaB p65 (NF-kappaB p65) inhibitor (IkappaB-alpha) thoracic aorta detected immunohistochemical assay, while expression ICAM-1 mRNA investigated situ hybridization. RESULTS SBP control significantly higher than that WKY (P < 0.05) accompanied upregulated mRNA, protein, NF-kappaB aortic endothelial cells (all P 0.01), IkappaB-alpha group lower 0.01). NaHS showed reduced downregulated ICAM-1, compared to untreated 0.05). In PPG, further increased decreased CONCLUSION might attenuate development hypertension through attenuating reactions.