Rapid sampling for single-cell analysis by capillary electrophoresis.
作者： Allison R. Nelson , Nancy L. Allbritton , Christopher E. Sims
DOI: 10.1016/S0091-679X(06)82026-1
关键词:
摘要: Single-cell analyses have found increasing importance in biological investigation. Recent technical advances made it possible to perform chemical separations of cellular constituents at the level individual cell. In this chapter, a laser-based method for rapid sampling living cells analysis using capillary electrophoresis is described. The platform technology consists ultrasensitive laser-induced fluorescence detection mated with microscope integrated pulsed Nd:YAG laser. This provides flexible system development new single-cell biochemical assays variety intracellular enzymes.
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