Measurement of kinase activation in single mammalian cells.
作者： Gavin D. Meredith , Christopher E. Sims , Joseph S. Soughayer , Nancy L. Allbritton
DOI: 10.1038/73760
关键词:
摘要: We demonstrate a new method for the simultaneous measurement of activation key regulatory enzymes within single cells. To illustrate capabilities technique, protein kinase C (PKC), A (PKA), calcium-calmodulin activated II (CamKII), and cdc2 (cdc2K) was measured in response to both pharmacological or physiological stimuli. This assay strategy should be applicable broad range intracellular enzymes, including phosphatases, proteases, nucleases, other kinases.
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