Influence of bacterial strain genotype on transient expression of plasmid DNA in plant protoplasts

摘要: It is demonstrated that transient expression of plasmid DNA in plant protoplasts can be strongly influenced by the bacterial strain used for propagation. Four different promoter constructs containing two light-responsive and fungal elicitor-responsive parsley promoters translationally fused to reporter gene, β-glucuronidase (GUS), were amplified strains MC1061, DH5α or GM2163 tested individually. Marked differences basal levels fold inducibilities observed upon transfection protoplasts. Low strong light elicitor only with derived from methylation-deficient strain. In vitro methylation prior transformation also drastically increased levels. The results suggest may partly responsible deregulating activity system.