Rotavirus A in Brazil: Molecular Epidemiology and Surveillance during 2018-2019.

摘要: Rotavirus A (RVA) vaccines succeeded in lowering the burden of acute gastroenteritis (AGE) worldwide, especially preventing severe disease and mortality. In 2019, Brazil completed 13 years RVA vaccine implementation (Rotarix™) within National Immunization Program (NIP), as reported elsewhere, use Rotarix™ country has reduced childhood mortality morbidity due to AGE. Even though both marketed are widely distributed, surveillance causing AGE monitoring circulating genotypes important tools keep tracking epidemiological scenario impact. Thus, our study investigated features, viral load G P circulation children adults presenting symptoms eleven states from three out five regions Brazil. By using TaqMan®-based one-step RT-qPCR, we a total 1536 stool samples collected symptomatic inpatients, emergency department visits outpatients January 2018 December 2019. RVA-positive were genetically characterized by multiplex RT-PCR or nearly complete fragment sequencing. We detected 12% samples, 10.5% 13.7% marked winter/spring seasonality was observed, Southern The most affected age group aged >24–60 months, with positivity rate 18.8% (p < 0.05). Evaluating shedding, found statistically lower up six months compared other groups genotype G3P[8] prevalent during two (83.7% 65.5% 2019), nucleotide sequencing some strains demonstrated that they belonged emergent equine-like genotype. dominance an reinforces need for continuous assess impact mass immunization well monitor emergence novel genotypes.