Inactivation conditions for human norovirus measured by an in situ capture-qRT-PCR method

摘要: Abstract Human norovirus (HuNoV) is a leading cause of foodborne gastroenteritis. Unfortunately, the inactivation parameters for HuNoV in clinical, food and environmental samples have not been established. Due to inability cultivate vitro, quantitative real-time RT-PCR (qRT-PCR) widely-used detecting HuNoVs. However, qRT-PCR does indicate viral infectivity. Our method employs histo-blood group antigens (HBGAs) as receptors/co-receptors container-affixed capture agents concentrate The captured viruses are denatured its genome amplified same module by situ (ISC-qRT-PCR). Greater than three log10 reduction receptor-captured genomic signal (RCVGS) was observed when treated heat at 72 °C 4 min, chlorine final concentration 16 ppm less 1 min, UV irradiation 1 J/cm2. Treatment low-titer (  103 copies/sample). results demonstrate that ISC-qRT-PCR could be used an alternative measure encapsidated RNA indirectly status caused physical treatment such heat, chemical chlorine, damage ability virus bind receptor.