Evaluation of methods measuring the capsid integrity and/or functions of noroviruses by heat inactivation
作者： Dan Li , Leen Baert , Ming Xia , Weiming Zhong , Els Van Coillie
DOI: 10.1016/J.JVIROMET.2012.01.001
关键词:
摘要: Attempts were made to evaluate methods measuring the capsid integrity and/or functions of noroviruses (NoVs) following heat treatment. Intact viruses (Murine Norovirus-1 [MNV-1] and human NoV GII.4), virus like particles (VLPs) P (expressed in vitro from protruding domain viral capsid) NoVs used this study. Following treatment, no significant difference titer MNV-1 versus GII.4 was observed by RNase One RT-PCR or cell-binding RT-PCR, although (to measure functions) revealed higher reductions than integrity). These results indicate that function assay for receptor binding is more sensitive protected RNA. could be as a surrogate inactivation perspective functions. The resistance varied among different GI GII strains when their studied.
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