Cellular neurochemical characterization and subcellular localization of phospholipase C β1 in rat brain.

作者: M. Montaña , G. García del Caño , M. López de Jesús , I. González-Burguera , L. Echeazarra

DOI: 10.1016/J.NEUROSCIENCE.2012.06.039

关键词:

摘要: The present study describes a complete and detailed neuroanatomical distribution map of the phospholipase C beta1 (PLCβ1) isoform along adult rat neuraxis, defines phenotype cells expressing PLCβ1, with its subcellular localization in cortical neurons as assessed by double-immunofluorescence staining confocal laser scanning. Immunohistochemical labeling revealed considerable morphological heterogeneity among PLCβ1-positive cortex, even though there was marked predominance pyramidal morphologies. As an exception to general non-matching GFAP high degree co-expression observed radial glia-like processes spinal cord white matter. In somatosensory proportion GABAergic co-stained PLCβ1 similar (around 2/3) layers I, II-III, IV VI, considerably lower layer V 2/5). Double immunofluorescence against nuclear speckle markers SC-35 NeuN/Fox3 isolated nuclei from cortex showed overlap both within matrix. contrast, no apparent co-localization envelope lamina. Finally, assess whether expression pattern involved specifically one two splice variants we carried out Western blot experiments fractions. Notably, PLCβ1a/1b ratios were statistically higher cytoplasm than plasma membrane These results provide deeper knowledge cellular different cell subtypes brain, presence neuronal compartment.

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