Specific glucose-to-SPR signal transduction at physiological pH by molecularly imprinted responsive hybrid microgels

摘要: We design a class of imprinted hybrid microgels that can optically monitor glucose levels with high sensitivity and selectivity in complex media at physiological pH, acting like "glucose-indicator". Such were made Ag nanoparticles (NPs) situ immobilized molecularly glucose-responsive polymeric microgel templates containing phenylboronic acids such way the NPs confined immediate vicinity to each other, thus enabling their efficient plasmon coupling. The gel-actuated tunable coupling effects among inside investigated both phosphate buffer solution artificial tear fluid. visually evident color shift from yellow red dispersion response concentration change 0 20.0 mm allows one see without instrumental aid. surface resonance (SPR) appropriate loading amount is free significant interferences major non-glucose constituents, optical sensing fluids achieved root-mean-squared error predication (RMSEC) as low 13.7 μM (~0.2 mg/dL) over clinically relevant range 0.1-20 (1.8-360 mg/dL). highly versatile could potentially be used for continuous monitoring clinical diagnostic bioprocess applications.