High-performance liquid chromatography of carthamin, safflor yellow A and a precursor of carthamin. Application to the investigation of an unknown red pigment produced in cultured cells of safflower.
作者： Katsuyuki Nakano , Yoshihiro Sekino , Naogoro Yomo , Sachio Wakayama , Shuji Miyano
DOI: 10.1016/S0021-9673(00)90233-4
关键词:
摘要: A reversed-phase high-performance liquid chromatographic (RP-HPLC) method was developed to analyse and purify carthamin, safflor yellow (safflomin-A) the precursor of carthamin. red pigment similar carthamin extracted from cell suspension cultures safflower (Carthamus tinctorius L.) as an application biotechnology. The RP-HPLC used characterize unknown pigment. Various spectroscopic methods were purified Based on these spectral observations, it concluded that produced in cultured cells differed although might be a glycoside. From other possibility anthocyanins carotenoids also discounted. It assumed lacked biosynthetic pathway they possessed activity enzyme mediating synthesis precursor.
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sci-hub.se 参考文章(8)
Toshio Murashige, Folke Skoog, A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures Physiologia Plantarum. ,vol. 15, pp. 473- 497 ,(1962) , 10.1111/J.1399-3054.1962.TB08052.X
K SAITO, Y TAKAHASHI, M WADA, Enzymic synthesis of carthamin in safflower Biochimica et Biophysica Acta. ,vol. 756, pp. 217- 222 ,(1983) , 10.1016/0304-4165(83)90094-6
Heitaro Obara, Jun-ichi Onodera, Structure of carthamin. Chemistry Letters. ,vol. 8, pp. 201- 204 ,(1979) , 10.1246/CL.1979.201
Yoshiyuki Takahashi, Nobutoshi Miyasaka, Shigeo Tasaka, Iwao Miura, Shiro Urano, Mitsuo Ikura, Kunio Hikichi, Takeshi Matsumoto, Mizu Wada, Constitution of two coloring matters in the flower petals of L. Tetrahedron Letters. ,vol. 23, pp. 5163- 5166 ,(1982) , 10.1016/S0040-4039(00)85786-X
Chika KURODA, Carthamin. I NIPPON KAGAKU KAISHI. ,vol. 51, pp. 237- 255 ,(1930) , 10.1246/NIKKASHI1921.51.237
Koshi Saito, Yoshiyuki Takahashi, Mizu Wada, Distribution and Variation of the Catalytic Activity of the Enzyme Responsible for Carthamin Synthesis in Safflower Seedlings Zeitschrift für Naturforschung C. ,vol. 38, pp. 724- 729 ,(1983) , 10.1515/ZNC-1983-9-1010
Heitaro Obara, Jun-ichi Onodera, CARTHAMIN AND ISOCARTHAMIN Chemistry Letters. ,vol. 7, pp. 643- 644 ,(1978) , 10.1246/CL.1978.643
Jun-ichi Onodera, Heitaro Obara, Masahide Osone, Yasunobu Maruyama, Shingo Sato, THE STRUCTURE OF SAFFLOMIN-A, A COMPONENT OF SAFFLOWER YELLOW Chemistry Letters. ,vol. 10, pp. 433- 436 ,(1981) , 10.1246/CL.1981.433