Bone Marrow Stromal Cell Paracrine Factors Direct Osteo/Odontogenic Differentiation of Dental Pulp Cells
作者： Niyaz Al-Sharabi , Ying Xue , Masahito Fujio , Minoru Ueda , Cecilie Gjerde
DOI: 10.1089/TEN.TEA.2013.0718
关键词:
摘要: Growth factors play an important role in osteo/odontogenic differentiation of human dental pulp cells (hDPCs). The aim this vitro study was to compare the biological effects recombinant growth factor 5 (rhGDF-5) alone and a cocktail soluble (conditioned medium) released from bone marrow mesenchymal stem (hBMMSCs) on morphology, proliferation potential hDPCs. Passage 4 hDPCs were harvested for culture four different media: (a) DMEM with 10% FBS, (b) odontogenic induction medium (OM), (c) OM plus 500 ng/mL rhGDF-5, (d) conditioned (CM). Morphological changes at 48 120 h determined by crystal violet staining. rates 3, 24, 48, assayed MTT. Using real-time reverse transcription-polymerase chain reaction (RT-PCR), mRNA levels dentin sialophosphoprotein (DSPP), matrix protein 1 (DMP1), collagen type I (Col 1), Runt-related tr...
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