TET1 knockdown inhibits the odontogenic differentiation potential of human dental pulp cells.
作者： Li-Jia Rao , Bai-Cheng Yi , Qi-Meng Li , Qiong Xu
DOI: 10.1038/IJOS.2016.4
关键词: Small hairpin RNA 、 Odontoblast 、 Cell growth 、 Cellular differentiation 、 Cell biology 、 Cell culture 、 DNA demethylation 、 Gene knockdown 、 DMP1 、 Pathology 、 Biology
摘要: Human dental pulp cells (hDPCs) possess the capacity to differentiate into odontoblast-like and generate reparative dentin in response exogenous stimuli or injury. Ten-eleven translocation 1 (TET1) is a novel DNA methyldioxygenase that plays an important role promotion of demethylation transcriptional regulation several cell lines. However, TET1 biological functions hDPCs unknown. To investigate effect on proliferation odontogenic differentiation potential hDPCs, recombinant shRNA lentiviral vector was used knock down expression hDPCs. Following knockdown, significantly downregulated at both mRNA protein levels. Proliferation suppressed knockdown groups. Alkaline phosphatase activity, formation mineralized nodules, levels DSPP DMP1 were all reduced TET1-knockdown undergoing differentiation. Based these results, we concluded can prevent which suggests may play repair regeneration.
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