Cellular effects of hematoporphyrin derivative photodynamic therapy on normal and neoplastic rat bladder cells.

摘要: HPD is known to localize in neoplastic cells and when exposed the appropriate wavelength of light causes cytotoxicity. The authors have established a rat urothelial cell model for use comparing contrasting effects photodynamic therapy (PDT) normal (RBL-01) transitional carcinoma (AY27) bladder lines. Uptake, toxicity, morphologic damage following exposure PDT were evaluated. Trypan blue exclusion was used determination toxicity several concentrations (1, 10, 25, 50 micrograms/ml) with increasing duration incubation (0, 1, 2, 4, 12, 24, 48 hours). Both lines displayed increased higher HPD; however, AY27 more susceptible toxic than RBL-01 at doses studied (25 micrograms/ml). Viability decreased up until 4 hours, after which it showed steady increase. incubation. An increase serum concentration medium resulted an viability both demonstrated fast initial uptake followed by slower over time studied. By 24 hours contained twice amount methanol-extractable porphyrins as cells. change mitochondria. Mitochondrial occurred immediately 30 minutes exhibited similar progression injury; observed earlier appeared extensive