Fusogenic virosomes prepared by partitioning of vesicular stomatitis virus G protein into preformed vesicles.

摘要: Virosomes were prepared by the insertion of vesicular stomatitis virus glycoprotein, a pH-sensitive fusion protein, into preformed liposomes. The fusogenic activity these virosomes was characterized in cell-free assays using liposomal targets. Fusion monitored concentration-dependent changes efficiency resonance energy transfer between N-(lissamine rhodamine B sulfonyl)-phosphatidylethanolamine and N-(4-nitrobenzo-2-oxa-1,3-diazol)-phosphatidylethanolamine electron microscopy. dependent on presence pH-sensitive, had pH threshold activation similar to that native virus. extent upon lipid composition vesicles. This technique will allow vesicles any method be made fusogenic.