Plasmon-Enhanced Single-Molecule Enzymology
作者： Yuyang Wang , Peter Zijlstra , None
DOI: 10.1021/ACSPHOTONICS.8B00327
关键词:
摘要: We present a numerical study on plasmon-enhanced single-molecule enzymology. combine Brownian dynamics and electromagnetic simulations to calculate the enhancement of fluorescence signals fluorogenic substrate converted by an enzyme conjugated plasmonic particle. simulate motion fluorescent product away from active site enzyme, photon detection rate taking into account modifications excitation emission processes coupling plasmon. show that plasmon can boost signal-to-noise ratio (SNR) single turnovers up 100 fold compared confocal microscopy. This factor is trade-off between reduced residence time in near-field particle, enhanced intensity due The depends size, shape material particle photophysical properties product. Our provides guidelines how enhance ...
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