Molecular characterization of the cell cycle-regulated thymidylate synthase gene of Saccharomyces cerevisiae.

摘要: Abstract The complete nucleotide sequence of a 1.8-kilobase DNA fragment containing the cell cycle-regulated thymidylate synthase gene (TMP 1) yeast Saccharomyces cerevisiae is presented. This analysis has revealed 912-base pair open reading frame which encodes 304-amino acid residue protein with calculated Mr 35,007. tmp1-6 and cdc21-1 mutant alleles this also have been sequenced, both show single base changes would result in different amino substitutions. derived from shows considerable homology when compared human, mouse, Herpesvirus saimiri, Leishmania major, tropica, Escherichia coli, Lactobacillus casei, bacteriophage T4, Bacillus subtilis phage phi 3T enzymes. Northern blot hybridization reveals that TMP 1 mRNA 1.15-kilobase polyadenylated transcript. A set consensus splice sequences appears within 1, but S1 nuclease protection experiments reveal splicing does not occur. Disruption by introduction large insertion did produce any defect besides expected dependence on dTMP for growth. Specifically, viability mutants presence indicates play significant structural role complex replication