Allergen-stimulated T lymphocytes from allergic patients induce vascular cell adhesion molecule-1 (VCAM-1) expression and IL-6 production by endothelial cells.

摘要: Adhesion of inflammatory cells to endothelium is a critical step for their transvascular migration sites. To evaluate the relationship between T lymphocytes (TL) and vascular endothelium, supernatants from allergen-stimulated TL obtained patients sensitive Dermatophagoides pteronyssinus (Dpt) versus healthy subjects were added endothelial cell (EC) cultures. stimulated by autologous-activated antigen-presenting (APC) previously fixed in paraformaldehyde prevent monokine secretion. Two parameters measured: expression adhesion molecule production IL-6. Related allergic induced an increase VCAM-1 intercellular molecule-1 (ICAM-1) when control groups (TL exposed unrelated allergen or not subjects) did not. E-selectin was modulated whatever supernatant EC culture. IL-6 significantly enhanced after activation with related allergics compared supernatants. Induction inhibited adding neutralizing antibodies against IL-4, whereas ICAM-1 anti-interferon-gamma (IFN-gamma) antibodies. Enhanced IL-4 IFN-gamma detected different These data suggest that allergen-specific present peripheral blood are Th1 Th2 subtypes. Their stimulation may lead thereby participate leucocyte recruitment towards site.