Electrokinetic bioprocessor for concentrating cells and molecules.

摘要: Bioprocessors for concentrating bioparticles, such as cells and molecules, are commonly needed in bioanalysis systems. In this microfluidic processor, a global flow field generated by ac electroosmosis transports the embedded particles to regions near electrode surface. The processor then utilizes electrophoretic dielectrophoretic forces, which effective short range, trap target molecules on By optimizing operating parameters, we have concentrated various biological objects large range of sizes, including Escherichia coli bacteria, λ phage DNA, single-stranded DNA fragments small 20 bases that radius gyration only 3 nm.