The Roles of CD81 and Glycosaminoglycans in the Adsorption and Uptake of Infectious HCV Particles

作者: Kenichi Morikawa , Zijiang Zhao , Tomoko Date , Michiko Miyamoto , Asako Murayama

DOI: 10.1002/JMV.20842

关键词:

摘要: Because appropriate cell-culture systems or small-animal models have been lacking, the early steps in HCV life cycle difficult to study. A cell culture system was developed recently that allows production of infectious HCV. In this study, particles produced cultured cells were used. To clarify role CD81 attachment and entry, effect anti-CD81 antibody examined. The blocked virion entry but not particle attachment. Only fraction bound a heparin affinity column eluted with 0.3 M NaCl productively infected Huh7 cells, indicating bind heparin. Both treatment virus heparinase reduced virus-cell binding without substantially inhibiting infectivity. Finally, confirm both sulfate proteoglycan (HSPG) effects I analyzed. No productive RNA replication detected presence antibody. conclusion, these data suggested HSPG are important for entry. may play initial surface is conceivably correlated after viral

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