Isolation of JFH-1 strain and development of an HCV infection system.

摘要: Detailed analysis of hepatitis C virus (HCV) has been hampered by the lack an appropriate viral culture system and small animal models infection. My group others have recently reported production infectious after full-length HCV RNA transfection into Huh-7 cells. This depends primarily on isolation a JFH-1 strain from patient with fulminant hepatitis. The belongs to genotype 2a high colony-formation efficiency when tested subgenomic replicon system. Here, I describe various protocols for construction infection contributes our understanding virology may permit development novel antiviral strategies.