Expression of human factors CD81, claudin-1, scavenger receptor, and occludin in mouse hepatocytes does not confer susceptibility to HCV entry
作者： Takaji Wakita , Naoyuki Miura , Keisuke Hikosaka , Hidenao Noritake , Wataru Kimura
关键词: Hepatitis C virus 、 Occludin 、 Receptor 、 Virus genetics 、 Biology 、 Virology 、 CD81 、 Antigen 、 Scavenger receptor 、 Genetically modified mouse
摘要: No suitable mouse model is available for studying chronic liver disease caused by hepatitis C virus (HCV). CD81, claudin-1, scavenger receptor class B type I, and occludin were recently reported to be the important factors in HCV entry into hepatocytes. We made transgenic mice (Alb-CCSO) expressing four human proteins examined whether from a patient serum or pseudoparticles (HCVpp) capable of infecting them. was not detected after injecting with serum. also found no indications HCVpp primary hepatocytes Alb-CCSO mice. In addition, HCV-infectible Hep3B cells fused HCV-resistant showed 35-fold lower infectivity compared wild-type cells, indicating that have inhibitory factor(s) entry. Our results suggest expression does confer susceptibility liver.
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