Viral Load Analysis of Hepatitis C Virus in Huh7.5 Cell Culture System
作者： Roghayeh Teimourpour , Zahra Meshkat , Aida Gholoubi , Hosein Nomani , Sina Rostami
DOI: 10.5812/JJM.8(5)2015.19279
关键词:
摘要: Background: Previous studies using cell culture systems for the replication of hepatitis C virus have opened new research dimensions, and paved ways further detailed in vitro. Objectives: The purpose present study was to cultivate a system evaluate viral amplification. Materials Methods: In order propagate virus, cloned whole genome JFH-1, used. JFH-1 cDNA introduced into strain JM109 Escherichia coli plasmid, containing purified from transformed bacteria. After XbaI digestion, RNA synthesis induced T7 polymerase enzyme. Next, eukaryotic line Huh 7.5 transfected by RNA. Finally, Huh-7.5 infected with replicated load determined real-time PCR (Polymerase Chain Reaction). Results: amount load, which measured 17592 IU/mL. Conclusions: study, culture, high titer (in acceptable range) infectious produced. This method could be used future studies.
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