Replication and multiplication of hepatitis C virus genome in human foetal liver cells
作者： S. Iacovacci , M. Sargiacomo , I. Parolini , A. Ponzetto , C. Peschle
DOI: 10.1016/S0923-2516(06)80040-3
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摘要: Summary The ability to obtain primary long-term cultures of human foetal hepatocytes maintaining liver differentiation characteristics in serum-free medium prompted us test their susceptibility hepatitis C virus infection. Using PCR, we detected the presence HCV RNA-positive strand supernatants and cells virus-infected hepatocyte cultures, at various times post-infection. Evidence effective genome replication multiplication was also based on time-dependent appearance putative RNA-negative strand, detection replicative intermediates an increase genomic templates HCV-infected cells.
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sci-hub.se 参考文章(13)
G. Carloni, S. Iacovacci, M. Sargiacomo, G. Ravagnan, A. Ponzetto, C. Peschle, M. Battaglia, Susceptibility of human liver cell cultures to hepatitis C virus infection Archives of virology. Supplementum. ,vol. 8, pp. 31- 39 ,(1993) , 10.1007/978-3-7091-9312-9_4
Gregory R. Reyes, Bahige M. Baroudy, Molecular Biology of Non-A, Non-B Hepatitis Agents: Hepatitis C and Hepatitis E Viruses Advances in Virus Research. ,vol. 40, pp. 57- 102 ,(1991) , 10.1016/S0065-3527(08)60277-8
Milagros Salas-Prato, Jean-Francois Tanguay, Yves Lefebvre, Don Wojciechowicz, H. Heng Liem, David W. Barnes, Ginette Ouellette, Ursula Muller-Eberhard, Attachment and multiplication, morphology and protein production of human fetal primary liver cells cultured in hormonally defined media In Vitro Cellular & Developmental Biology – Plant. ,vol. 24, pp. 230- 238 ,(1988) , 10.1007/BF02623552
T L Fong, M Shindo, S M Feinstone, J H Hoofnagle, A M Di Bisceglie, Detection of replicative intermediates of hepatitis C viral RNA in liver and serum of patients with chronic hepatitis C. Journal of Clinical Investigation. ,vol. 88, pp. 1058- 1060 ,(1991) , 10.1172/JCI115368
E. S. Kawasaki, S. S. Clark, M. Y. Coyne, S. D. Smith, R. Champlin, O. N. Witte, F. P. McCormick, Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 85, pp. 5698- 5702 ,(1988) , 10.1073/PNAS.85.15.5698
R. Saiki, D. Gelfand, S Stoffel, S. Scharf, R Higuchi, G. Horn, K. Mullis, H. Erlich, Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase Science. ,vol. 239, pp. 487- 491 ,(1988) , 10.1126/SCIENCE.2448875
Shin Takeda, Motohiro Shibata, Tsuneo Morishima, Akio Harada, Akimasa Nakao, Hiroshi Takagi, Yoshiyuki Nagai, Hepatitis C virus infection in hepatocellular carcinoma. Detection of plus-strand and minus-strand viral RNA. Cancer. ,vol. 70, pp. 2255- 2259 ,(1992) , 10.1002/1097-0142(19921101)70:9<2255::AID-CNCR2820700907>3.0.CO;2-N
Y. K. Shimizu, A. Iwamoto, M. Hijikata, R. H. Purcell, H. Yoshikura, Evidence for in vitro replication of hepatitis C virus genome in a human T-cell line. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 89, pp. 5477- 5481 ,(1992) , 10.1073/PNAS.89.12.5477
L. Bertolini, S. Iacovacci, A. Ponzetto, G. Gorini, M. Battaglia, G. Carloni, The human bone-marrow-derived B-cell line CE, susceptible to hepatitis C virus infection Research in Virology. ,vol. 144, pp. 281- 285 ,(1993) , 10.1016/S0923-2516(06)80041-5
P Simmonds, S Rebus, G.H Leadbetter, J.F Peutherer, L.Q Zhang, P Balfe, E.D Ferguson, H.G Watson, C.A Ludlam, P.L Yap, Hepatitis C quantification and sequencing in blood products, haemophiliacs, and drug users. The Lancet. ,vol. 336, pp. 1469- 1472 ,(1990) , 10.1016/0140-6736(90)93179-S