[HCV replication in Huh-7.5 cell line].

摘要: Introduction Infection with hepatitis C virus is a serious worldwide health problem. Since its discovery in 1989, the development of cell culture system for HCV has been major goal scientists worldwide. In 2005 first tissue that led to production particles (2a genotype) created. The aim study was determine viral RNA level an infectious system. Methods Huh-7.5 line infected JFH1 (Japanese Fulminant Hepatitis) by lipofection. measured supernatant Real-Time PCR method. Results detected use ofJFH 1 and lipofectamin. maximal (3.69 x 10(9) copies/ml) 96h after transfection. After about 30 days oftransfection, on stable (10(7)-10(8)). Conclusions form robust model infection. replicates high levels Huh-7.5, which crucial event investigation new antivirals vitro model.