Novel Cell Culture-Adapted Genotype 2a Hepatitis C Virus Infectious Clone
作者： T. Date , T. Kato , J. Kato , H. Takahashi , K. Morikawa
DOI: 10.1128/JVI.07235-11
关键词: Biology 、 Mutation 、 Virology 、 Resistance mutation 、 Hepatitis C virus 、 Clone (cell biology) 、 Oncovirus 、 Virus 、 NS5A 、 Replicon
摘要: Although the recently developed infectious hepatitis C virus system that uses JFH-1 clone enables study of whole HCV viral life cycles, limited particular strains have been available with system. In this study, we isolated another genotype 2a cDNA, JFH-2 strain, from a patient fulminant hepatitis. subgenomic replicons were constructed. HuH-7 cells transfected in vitro transcribed replicon RNAs cultured G418, and selected colonies expanded. From sequencing analysis genome, several mutations found. Some enhanced replication; 2217AS mutation NS5A interferon sensitivity-determining region exhibited strongest adaptive effect. Interestingly, full-length chimeric or wild-type genome could replicate Huh-7.5.1 produce after extensive passages genome-replicating cells. Virus infection efficiency was sufficient for autonomous propagation Additional identified genome. RNA synthesized cDNA these This approach may be applicable establishment new clones.
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sci-hub.se 参考文章(39)
Takaji Wakita, Isolation of JFH-1 strain and development of an HCV infection system. Methods of Molecular Biology. ,vol. 510, pp. 305- 327 ,(2009) , 10.1007/978-1-59745-394-3_23
Takanobu Kato, Tomoko Date, Michiko Miyamoto, Akihiro Furusaka, Katsutoshi Tokushige, Masashi Mizokami, Takaji Wakita, Efficient replication of the genotype 2a hepatitis C virus subgenomic replicon Gastroenterology. ,vol. 125, pp. 1808- 1817 ,(2003) , 10.1053/J.GASTRO.2003.09.023
M. Yanagi, R. H. Purcell, S. U. Emerson, J. Bukh, Transcripts from a single full-length cDNA clone of hepatitis C virus are infectious when directly transfected into the liver of a chimpanzee Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 8738- 8743 ,(1997) , 10.1073/PNAS.94.16.8738
Adrian M. Di Bisceglie, Jay H. Hoofnagle, Optimal therapy of hepatitis C Hepatology. ,vol. 36, pp. S121- S127 ,(2002) , 10.1002/HEP.1840360716
Asako Murayama, Leiyun Weng, Tomoko Date, Daisuke Akazawa, Xiao Tian, Tetsuro Suzuki, Takanobu Kato, Yasuhito Tanaka, Masashi Mizokami, Takaji Wakita, Tetsuya Toyoda, RNA Polymerase Activity and Specific RNA Structure Are Required for Efficient HCV Replication in Cultured Cells PLOS Pathogens. ,vol. 6, ,(2010) , 10.1371/JOURNAL.PPAT.1000885
Masayuki Yanagi, Robert H. Purcell, Suzanne U. Emerson, Jens Bukh, Hepatitis C virus: an infectious molecular clone of a second major genotype (2a) and lack of viability of intertypic 1a and 2a chimeras. Virology. ,vol. 262, pp. 250- 263 ,(1999) , 10.1006/VIRO.1999.9889
Takaji Wakita, Thomas Pietschmann, Takanobu Kato, Tomoko Date, Michiko Miyamoto, Zijiang Zhao, Krishna Murthy, Anja Habermann, Hans-Georg Kräusslich, Masashi Mizokami, Ralf Bartenschlager, T Jake Liang, Production of infectious hepatitis C virus in tissue culture from a cloned viral genome Nature Medicine. ,vol. 11, pp. 791- 796 ,(2005) , 10.1038/NM1268
Q. Choo, G Kuo, A. Weiner, L. Overby, D. Bradley, M Houghton, Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome Science. ,vol. 244, pp. 359- 362 ,(1989) , 10.1126/SCIENCE.2523562
Daisuke Akazawa, Kenichi Morikawa, Noriaki Omi, Hitoshi Takahashi, Noriko Nakamura, Hidenori Mochizuki, Tomoko Date, Koji Ishii, Tetsuro Suzuki, Takaji Wakita, Production and characterization of HCV particles from serum-free culture Vaccine. ,vol. 29, pp. 4821- 4828 ,(2011) , 10.1016/J.VACCINE.2011.04.069
B. D. Lindenbach, P. Meuleman, A. Ploss, T. Vanwolleghem, A. J. Syder, J. A. McKeating, R. E. Lanford, S. M. Feinstone, M. E. Major, G. Leroux-Roels, C. M. Rice, Cell culture-grown hepatitis C virus is infectious in vivo and can be recultured in vitro Proceedings of the National Academy of Sciences of the United States of America. ,vol. 103, pp. 3805- 3809 ,(2006) , 10.1073/PNAS.0511218103