The structure of the cell wall of the Gram-negative bacterium Proteus vulgaris. I. An electron microscope and x-ray study.

摘要: The structure of the cell wall Gram-negative bacterium Proteus vulgaris has been investigated in this work by electron microscopy and low- high-angle X-ray diffraction. walls were characterized studies on whole cells subsequent observations made oriented preparations isolated components. effects staining (e.g. with uranyl acetate), fixation embedding procedures osmium-methacrylate, permanganate-Araldite) involved preparation specimens for examined These results are discussed connection other authors from bacteria. In addition described both short prolonged extraction a lipid solvent. is known to comprise rigid mucopeptide-protein layer or R interpenetrating lipopolysaccharide lipoprotein layers. data untreated, fixed stained show simultaneous existence varying proportions two sheet-like structures respective thicknesses 92 144 A. A component shown have familiar triple-layered “unit membrane” appearance identified lacking labile layer. was found only difficulty micrographs walls, complete wall. loss superficial reduces leaflet 70 thick which most, if not all, dense-light-dense divisions triple also extracted. microscope taken together allow assignment dimensions various density layers relationships these measurements previously published biochemical, microscopic discussed.