Adhesion pattern and growth of primary human osteoblastic cells on five commercially available titanium surfaces.

摘要: Objective The aim of this study is to analyze the morphology and proliferation human osteoblastic cells in vitro on five commercially available titanium surfaces. Materials methods Human primary lineage were obtained from bone explants. plated polished (T1), machined (T2), sand-blasted/acid-etched (T3), sand-blasted/acid-etched, modified with hydrogen peroxide rinse (T4), plasma-sprayed (T5) disks. Cell was studied after 6, 24, 72 h, 7 14 days culture by scanning electron microscopy. formation distribution focal adhesions investigated immunocytochemical staining at 3, 6 24 h. growth measured an MTT assay 9 culture. Moreover, production osteocalcin osteoprotegerin (OPG) evaluated supernatants ELISA. Results Morphological analysis revealed that substrate topography profoundly affected cells' shape their anchoring structures. Large lamellipodia formed surfaces, while thin filopodia more frequently observed T3 T4 samples. stronger cell higher rough Osteocalcin surface, whereas OPG steadily increased every surface. Conclusions Taken together, these data show all surfaces allowed attachment, adhesion proliferation, but T5 appeared be a better for adhesion, differentiation lineage.