Differential L chain expression in the antibody responses to phosphorylcholine of adult bone marrow or peritoneum-derived B lymphocytes.

摘要: Lethal irradiation of adult BALB/c mice followed by reconstitution with autologous bone marrow results in loss T15 Id and IdX expression the responses to phosphorylcholine (PC) alpha(1-3)-dextran, respectively. Id, but not can be reconstituted low numbers syngeneic, T cell-depleted peritoneal resident cells. All three groups produce comparable titers specific anti-PC anti-dextran antibodies. The inability marrow-reconstituted Id+ antibodies is due differential VH-gene or peritoneum-derived B Thus, levels VH total serum Ig are similar all mice. Furthermore, IEF patterns VH-associated L chains directly demonstrate Vk repertoire